A microtitre plate assay for characterizing insensitive acetylcholinesterase genotypes of insecticide-resistant insects
نویسندگان
چکیده
منابع مشابه
Resistance-associated point mutations in insecticide-insensitive acetylcholinesterase.
Extensive utilization of pesticides against insects provides us with a good model for studying the adaptation of a eukaryotic genome to a strong selective pressure. One mechanism of resistance is the alteration of acetylcholinesterase (EC 3.1.1.7), the molecular target for organophosphates and carbamates. Here, we report the sequence analysis of the Ace gene in several resistant field strains o...
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The ability of Prevotella intermedia to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. All strains of P. intermedia tested, as well as those of the closely related species Prevotella nigrescens, showed a capacity to attach to the collagen film. Exponential-pha...
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AIMS To develop a DNA based plate hybridisation assay for the detection of polymerase chain reaction (PCR) products amplified from Aspergillus fumigatus DNA; and to determine the sensitivity of this technique and compare it with Southern blotting. METHODS A half-log dilution series of DNA extracted from A fumigatus was amplified with specific primers, one of which was 5' end labelled with bio...
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The exploration of new niches for microorganisms capable of degrading recalcitrant molecules is still required. We hypothesized the gut microbiota associated with insect-resistant lines carry pesticide degrading bacteria, and predicted they carry bacteria selected to degrade pesticides they were resistant to. We isolated and accessed the pesticide-degrading capacity of gut bacteria from the gut...
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A kinetic assay for alanine transferase (ALT) using microtitre plates and commercially available reagents was developed to retain the advantages conferred by multichannel and semiautomated equipment. The time taken to assay 92 samples was less than 10 minutes, and costs of apparatus and reagents compared favourably with most current methods. Although reactions became non-linear above 120 IU/l-1...
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ژورنال
عنوان ژورنال: Bulletin of Entomological Research
سال: 1988
ISSN: 0007-4853,1475-2670
DOI: 10.1017/s0007485300013286